PCR is central to short read sequencing... as well as Roche 454, and can also be used in some protocols in Nanopore.

https://pmc.ncbi.nlm.nih.gov/articles/PMC3849550/

You can sequence a human genome on a MinION - but you need to purchase 5 flow cells to reach 11X (if they are used correctly).. https://nanoporetech.com/news/news-human-genome-minion

> PCR [...] can also be used in some protocols in Nanopore.

Yes... and that is why I said that the presence of PCR does not imply short reads.

> You can sequence a human genome on a MinION - but you need to purchase 5 flow cells to reach 11X (if they are used correctly).. https://nanoporetech.com/news/news-human-genome-minion

THat's why I said that 5/20/30X coverage is possible in the appropriate research setting

tellmeGen have begun to offer it in Spain... 30X for £269 with 10% discount on now with coupon HALLOWEEN10 until Nov 4th.

https://shop.tellmegen.com/en/collections/ultra

oddly enough I just was looking at someone's data with chr13:20189491 A>G (gnomAD genomes v4 AF=0.00941) - also 0/1 genotype.

I used WGS from Nebula - but would like to back that up with Nanopore raw DNA reads targeted on specific genes where I need more accuracy and to investigate structural differences that Illumina or Nebula's MGI machines can't pick up... also for the additional methylation data.

Now you can buy a portable thermocycler with 13k rpm centrifuge all-in-one with gel electrophoresis - BentoLab for £1299 in UK. Carry case £54. (They sell a Dipstick DNA Extraction kit for £38.50, pipettes, and mastermix...)

A portable lab with a thermocycler, 13k rpm centrifuge, and gel electrophoresis https://bento.bio/bento-lab/